Fluorescence Lifetime Imaging (FLIM) in Confocal Microscopy Applications: An Overview
نویسندگان
چکیده
During the last two decades, the use of fluorescence in natural sciences has propelled a number of research fields, ranging from biological sciences and applications in the clinical sector (FISH for genetic testing, advanced sequencing technology) to environmental monitoring. In addition, the resulting technological developments have become important in several fields of materials sciences, for example in characterization of new materials or quality control of semiconductor materials.
منابع مشابه
Fluorescence-Lifetime Imaging Microscopy for Visualization of Quantum Dots’ Endocytic Pathway
Accumulation of carboxylated polyethylene glycol (PEG) CdSe/ZnSquantum dots (QDs) has been monitored in living fibroblasts using confocal microscopy for fluorescence intensity and fluorescence-lifetime imaging (FLIM). The wide range of mean photoluminescence (PL) lifetime values was observed for the intracellular QDs in different intracellular microenvironment, which revealed structural heterog...
متن کاملFluorescence intensity and lifetime imaging of free and micellar-encapsulated doxorubicin in living cells.
Frequency domain fluorescence lifetime imaging microscopy (FLIM) has been used in combination with laser scanning confocal microscopy to study the cellular uptake behavior of the antitumor drug doxorubicin (DOX) and micellar-encapsulated DOX (PLyAd-DOX). The endocytosis uptake process of PLyAd-DOX was monitored over 72 hours using confocal microscopy, with a maximum fluorescence recorded at inc...
متن کاملHigh-speed fluorescence lifetime imaging microscopy for multimodal visualization of biomedical characteristics using a commercial microscope platform
Fluorescence lifetime imaging microscopy (FLIM) is one of the most powerful technique to visualize various photo-physical characteristics of the specimen [1]. Since it is sensitive to various chemical properties such as pH, ion concentration, or molecular interactions, FLIM has been applied to analyze various biomedical effects [2]. However, the application of FLIM into live specimen is usually...
متن کاملMultiphoton Microscopy for Ophthalmic Imaging
We review multiphoton microscopy (MPM) including two-photon autofluorescence (2PAF), second harmonic generation (SHG), third harmonic generation (THG), fluorescence lifetime (FLIM), and coherent anti-Stokes Raman Scattering (CARS) with relevance to clinical applications in ophthalmology. The different imaging modalities are discussed highlighting the particular strength that each has for functi...
متن کاملFluorescence lifetime imaging and reflectance confocal microscopy for multiscale imaging of oral precancer.
Optical imaging techniques using a variety of contrast mechanisms are under evaluation for early detection of epithelial precancer; however, tradeoffs in field of view (FOV) and resolution may limit their application. Therefore, we present a multiscale multimodal optical imaging system combining macroscopic biochemical imaging of fluorescence lifetime imaging (FLIM) with subcellular morphologic...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2014